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C6-P:MALDI-TOF MASS SPECTROMETRY AS A METHOD FOR THE QUALITY CONTROL
OF GENTIANA LUTEA EXTRACTS

B. Nastasijević, T. Lazarević Pašti, B. Damnjanović, T. Kamčeva, G. Joksić, V. Vasić

Vinča Institute of Nuclear Sciences, Belgrade, Serbia

 

Gentiana lutea is perennial herb commonly growing in the mountain areas of central and southern Europe. Gentiana radix, official drug in medicines, is actually dried fermented rhizomes and roots of Gentiana. Traditionally, this herb is used for the treatment of gastrointestinal disorders. MALDI-TOF mass spectrometry has been chosen as an additional method in quality control of herbal extract, beside known and approved method which is in use for these purposes. In this study methanol extract of Gentiana lutea was investigated. First step in quality control and then in identification of this herb was separation of methanol extract by UPLC chromatography, with UV detection. Developed gradient allowed an efficient separation of at least 10 compounds within the methanol extracts. At the same time it also represents characteristic fingerprint chromatogram for this herb. After UPLC separation, fractions were collected and analyzed by MALDI-TOF MS. CHCA and DHB have been used as matrix. Monitored m/z mass: 243,53; 338,96; 430,83 and 437,37 were confirmed presence of Gentisine, Gentiopicroside (characteristic for Gentiana lutea) as well Isovitexin and belidifolin-8-O-glucoside, characteristic for family of Gentianaceae. These results suggest that MALDI-TOF MS could be used as reliable analytical method together with UPLC fingerprinting chromatography, which is already established method by World Health Organization WHO, for herb extract authentication.

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