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B18-P: APPLICATION OF TIME RESOLVED LASER-INDUCED FLUORESCENCE MEASUREMENTS AND LASER INDUCED BREAKDOWN SPECTROSCOPY
FOR DEVELOPMENT OF NEW METHODS FOR FOOD QUALITY CONTROL

B. Marinkovic (1), M. Rabasovic (1), M. Terzic (2), D. Sevic (1)

1 Institute of Physics, Belgrade, Serbia
2 Faculty of Science, University of Novi Sad, Serbia

 

The aim of this presentation is to show possibilities of use of combined time resolved laser induced fluorescence (TR-LIF) and laser induced breakdown spectroscopy (LIBS) system for development of new methods for food quality control. The TRLIF/LIBS system is implemented in our laboratory.

Time-resolved laser-induced fluorescence (TR-LIF) has been shown to be a method which is fast and sensitive to provide information about the proteins and molecules significant for bioanalytical and chemical processes. Capabilities of TR-LIF/LIBS system implemented in our laboratory will be illustrated by presenting some of images acquired in the process of tuning the system.

A number of studies have appeared on the detection, spectroscopy and lifetime measurements of organic molecules based on laser-induced fluorescence (LIF) techniques [1, 2]. This technique is one of the most sensitive approaches in research for a variety of analytical applications in the fields of life sciences, biophysics and biomedical applications [3, 4]. LIBS technique could be useful for detection of toxic elements in food.

A detailed description and some of the preliminary results of our TRLIF/LIBS are given in [5-8]. Shortly, pulsed excitation is provided by a tunable Nd-YAG laser system (Vibrant model 266 made by Opotek, Inc.) with pulse duration of 5.4 ns, pulse repetition rate of 10 Hz and energy per pulse of up to~350 mJ. This system incorporates the optical parametric oscillator (OPO) that is pumped by the fourth harmonics of the Nd:YAG Brilliant laser at 266 nm. The output of the OPO can be continuously tuned over a spectral range from 320 nm to 475 nm. The laser induced fluorescence in the samples is recorded using streak scope (Hamamatsu model C4334-01) with integrated video streak camera. The fundamental advantage of the streak scope is its two dimensional nature, that is especially important in measuring time-resolved fluorescence spectra. The data have been acquired using HPD-TA software.

References

1. J. Lakowics, “Principles of Fluorescence Spectroscopy”, Springer- Verlag, New York, 2006.
2. N. Bras, Laser Chem. 1990, 10, 405-412.
3. K. Dowling, M. J. Dayel, S. C. W. Hyde, P. M. W. French, J. Mod. Opt. 1999, 46, 199-209.
4. M. Ishikawa, M. Watanabe, T. Hayakawa, M. Koishi, Anal. Chem. 1995, 67, 511-518.
5. M. Terzic, B.P. Marinkovic, D. Sevic, J. Jureta, A.R. Milosavljevic, Facta Universitatis, Series Phys. Chem. Technol. 2008, 6, 105-117.
6. M.S. Rabasovic, D. Sevic, M. Terzic, S.Savic-Sevic, B. Muric, D. Pantelic and B.P. Marinkovic, Acta Physica Polonica A 2009,  116  570-572.
7. M.S. Rabasovic, D. Sevic, M. Terzic, B.P. Marinkovic, Proceedings of SPIG 2010.
8. M.S. Rabasovic, D. Sevic, M. Terzic, B.P. Marinkovic, Proceedings of ECAMP 2010.