OP B8. BIOSENSOR FOR MONITORING ORGANOPHOSPHATE
AND CARBAMATE PESTICIDES IN WATER AND FRUIT JUICE
V. Vasić, M. Čolović, D. Krstić, J. Savić
Vinča Institute of Nuclear Sciences, Belgrade, Serbia
and
M. Franko, P. Trebše, M. Bavcon Kralj
University of Nova Gorica, Nova Gorica, Slovenia
Organophosphorous and carbamate pesticides, which show low environmental persistence but high acute toxicity, are often present in water and food. Pesticides and their breakdown products may become hazardous to human health, directly as in the spray drift of pesticides in edible plant or animal tissues. Therefore the growing interest exists in their rapid and accurate monitoring in food, water and in air and soil. The innovative analytical procedure to be introduced on Serbian market is the biochemical sensor based on AChE enzymatic assay, which offers advantages such as the possibility to be incorporated in miniaturized and portable analytical device for in-field and/or on line measurements of pesticides from organophosphate and carbamate group. Biosensor consists of a) biological component- bioanalytical column (immobilized enzyme packed in stainless steal column) which is selectively sensitive (on the level of molecular recognition) to target analytes; b) transducer which transforms biological component - target analyte interaction in to the optical signal, proportional to target analyte concentration and c) detector that automatically identifies and records or registers a signal. The major and the most important component of biosensor device is bioanalytical column with immobilized enzymes from cholinesterase group. The prototype of biosensor was constructed by coupling the bioanalytical column into a FIA system with the colorimeter spectrophotometer as a detector. The enzyme acetilcholinesterase was immobilized on solid support and the optimal conditions of packing of immobilized enzyme in to the column were defined. The reproducibility of immobilization method was investigated and the optimal working conditions for application of bioanalytical system to detect organophosphates and carbamates in water and fruit juice were determined. Validation and verification of biosensor prototype was established in the users facilities, where it was applied for detection of pesticides in water and fruit juice. The results were compared with those obtained by standard methods for analyze the same tested samples. |
