P A8. THE SWINE MEAT SAFETY - APPLICATION OF THE MEAT
JUICE IN DETECTION OF T. SPIRALIS INFECTION AT SLAUGHTER-HOUSES

M. Petrović 1,  M. Djordjević2, N. Ilić 1, and Lj. Sofronić-Milosavljević 1

1 Institute for the Application of  Nuclear Energy “INEP”, University of Belgrade, Banatska 31b, 11080 Belgrade, Serbia; 
2 Institute of Meat Hygiene and Technology “IMHT”, Kaćanskog 13, 11 000 Belgrade, Serbia

Pooled digestion is direct parasitological method obligatory to perform in slaughter-houses for Trichinella meat inspection. If presence of parasite is confirmed in the pool of meet samples, a time consuming process of re-examination takes place for identification of individually infected swine. The aim of this study was to investigate if indirect tests (for specific antibody detection) could be applied as intermediate method to reduce time of long lasting, Trichinella positive pool sample re-examination. For this purpose double testing of meat juice samples originating from T. spiralis infected and non-infected pig carcasses, was performed by ELISA and lateral flow  “TS Card Pork” (TSCP) tests. The confidential level of pooled digestion method of 1 gram meat sample and ELISA was 92,5%, while for TSCP test it was 100%. This indicates that results obtained by TSCP, could help in significant reduction of number of parasitological analyses. Consequently the time for re-examination will be reduced and expenses for safe meat production decreased.